Ayapana triplinervis is a plant species widely used in healing processes and in mystical-religious rituals by traditional communities in South America, Africa and Asia. This study aims to make a systematic review of the ethnopharmacological employment, secondary metabolites and biological activity reported in the scientific literature for A. triplinervis. The systematic literature review included scientific articles and theses published between 1987 and 2021 in Google Scholar, Science Direct, Pubmed, Web of Knowledge and CAPES Periodicals databases in English, Portuguese and Spanish. The results indicated that A. triplinervis is chemodiverse in coumarins and validated the ethnopharmacological action cited by traditional communities as antiviral, antinociceptive, antiulcerative, anxiolytic, biocidal activities by in vitro and in vivo biological tests.
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