High-performance Liquid Chromatography Analysis of Plant Saponins: An update 2005-2010

Pharmacognosy Review,2011,5,10,155-158.
DOI:10.4103/0973-7847.91109
Published:December 2011
Type:Review Articles
Authors:
Author(s) affiliations:

Jagmohan S Negi1-2, Pramod Singh1, Geeta Joshi Nee Pant1, M.S M Rawat1
1Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal); Herbal Research and Development Institute, Gopeshwar (Chamoli), Uttarakhand, India
2Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal), India

Abstract:

Saponins are widely distributed in plant kingdom. In view of their wide range of biological activities and occurrence as complex mixtures, saponins have been purified and separated by high-performance liquid chromatography using reverse-phase columns at lower wavelength. Mostly, saponins are not detected by ultraviolet detector due to lack of chromophores. Electrospray ionization mass spectrometry, diode array detector , evaporative light scattering detection, and charged aerosols have been used for overcoming the detection problem of saponins.

Keywords:Charged Aerosol, Diode Array Detector, ESI, Evaporative Light Scattering Detection, High-Performance Liquid Chromatography, Mass Spectrometry, Mobile Phase, Potodiode Array Detectors, Sapogenins, Saponins, Ultraviole

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Cite This Article

Vancouver Style ::
J. S. Negi, Singh, P. , Pant, G. Joshi Nee, and Rawat, M. S. M. , “High-performance Liquid Chromatography Analysis of Plant Saponins: An update 2005-2010”, Pharmacognosy Review, vol. 5, no. 10, pp. 155-158, 2011.

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